System and method for performing G protein coupled receptor (GPCR) cell assays using waveguide-grating sensors

ABSTRACT

The present invention includes a system and method that uses optical LID biosensors to monitor in real time agonist-induced GPCR signaling events within living cells. Particularly, the present invention includes a system and method for using an optical LID biosensor to screen compounds against a target GPCR within living cells based on the mass redistribution due to agonist-induced GPCR activation. In an extended embodiment, the present invention discloses different ways for self-referencing the optical LID biosensor to eliminate unwanted sensitivity to ambient temperature, pressure fluctuations, and other environmental changes. In yet another extended embodiment, the present invention discloses different ways for screening multiple GPCRs in a single type of cell or multiple GPCRs in multiple types of cells within a single medium solution. In still yet another extended embodiment, the present invention discloses different ways to confirm the physiological or pharmacological effect of a compound against a specific GPCR within living cells.

BACKGROUND OF THE INVENTION

1. Field of the Invention

The present invention relates in general to the optical non-contactsensor field and, specifically, to a system and method for using anoptical label independent detection (LID) biosensor (e.g., waveguidegrating-based biosensor) to monitor in real time compound-induced massredistribution in living cells, including agonist-induced G proteincoupled receptor (GPCR) desensitization and translocation within livingcells, as well as morphological changes of adherent cells. Particularly,the present invention relates to a system and method for using a LIDbiosensor to screen compounds against a GPCR within living cells.

2. Description of Related Art

Today an optical-based biosensor like a surface plasmon resonance (SPR)sensor or a waveguide grating-based sensor enables an optical labelindependent detection (LID) technique to be used to detect abiomolecular binding event at the biosensor's surface. In particular,the optical-based biosensor enables an optical LID technique to be usedto measure changes in a refractive index/optical response of thebiosensor which in turns enables a biomolecular binding event to bedetected at the biosensor's surface. In fact, these optical-basedbiosensors along with different optical LID techniques have been used tostudy a variety of biomolecular binding events includingoligonucleotides interactions, antibody-antigen interactions,hormone-receptor interactions, and enzyme-substrate interactions (forexample).

In general, the optical-based biosensor includes two components: ahighly specific recognition element and an optical transducer thatconverts a molecular recognition event into a quantifiable signal. Thetraditional studies performed with optical LID techniques have beenassociated with direct optical methods which include the use of: surfaceplasmon resonance (SPR) sensors; grating couplers; ellipsometry devices;evanescent wave devices; and reflectometry devices. For a detaildiscussion about each of these direct optical methods reference is madeto the following documents:

-   -   Jordan & Corn, “Surface Plasmon Resonance Imaging Measurements        of Electrostatic Biopolymer Adsorption onto Chemically Modified        Gold Surfaces,” Anal. Chem., 1997, 69:1449-1456.    -   Morhard et al., “Immobilization of antibodies in micropatterns        for cell detection by optical diffraction,” Sensors and        Actuators B, 2000, 70, 232-242.    -   Jin et al., “A biosensor concept based on imaging ellipsometry        for visualization of biomolecular interactions,” Analytical        Biochemistry, 1995, 232, 69-72.    -   Clerc and Lukosz “Direct immunosensing with an        integrated-optical output grating coupler” Sensors and Actuators        B 1997, 40, 53-58.    -   Brecht & Gauglitz, “Optical probes and transducers,” Biosensors        and Bioelectronics, 1995, 10, 923-936.        The contents of these documents are incorporated by reference        herein.

To date, there have been relatively few reports describing the use ofoptical LID techniques to perform cell-based assays. For example, SPRbiosensors have been used to investigate the adhesion and spreading ofanimal cells as described in the following document:

-   -   J. J Ramsden, S. Y. Li, J. E. Prenosil and E. Heinzle, “Kinetics        of adhension and spreading of animal cells” Biotechnol. Bioeng.        1994, 43, 939-945.

And, SPR biosensors have been used to investigate ligand-induced cellsurface and intracellular reactions of living cells as described in thefollowing document:

-   -   M. Hide, et al. “Real-time analysis of ligand-induced cell        surface and intracellular reactions of living mast cells using a        surface plasmon resonance-based biosensor”, Anal. Biochem. 2002,        302, 28-37.

However, to date there has been no report concerning the use of opticalLID techniques to monitor compound-induced mass redistribution withinadherent cells including agonist-induced translocation of G proteincoupled receptors (GPCRs) within living cells. It would be desirable ifthis was possible, because GPCRs, a family of cell surface receptors,are the most common targets that new drug compounds are designedagainst. And, because GPCRs can transduce exogenous signals (i.e., thepresence of stimuli such as a new drug) into intracellular response(s)which makes them extremely valuable in the testing of new drugs.

GPCRs participate in a wide array of cell signaling pathways. Ligandbinding initiates a series of intracellular and cellular signalingevents, including receptor conformational changes, receptoroligomerization, G protein activation (GDP-GTP exchanges on G_(α)subunit, G_(α) and G_(βγ) disassociation, G protein decoupling from thereceptor, generation of G_(α)- and G_(βγ)-signaling complexes), anddownstream signaling activation that leads to second messengergeneration (Ca²⁺ mobilization, inositoltriphosphate generation, and/orintracellular cAMP level modulation) and ultimately results in changesof specific gene expression. Ligand-mediated GPCR activation also leadsto the desensitization of GPCRs from the cell surface and trafficking ofmany intracellular proteins, as well as changes in phenotypes,morphology and physical properties of the target cells. These changescould be static, long-lasting or dynamic (e.g., cycling or oscillation).Distinct signaling events exhibit significantly different kineticsranging from milliseconds (e.g., GPCR conformational changes) to tens ofseconds (e.g., Ca²⁺ flux) to even tens of minutes (e.g., geneexpression, or morphological changes). Current GPCR assays includeligand-receptor binding, second messenger (Ca²⁺, cAMP of IP3) assays,protein interaction assays, translocation assays and reporter geneassays. Since GPCR activation ultimately leads to protein traffickingand/or morphological changes, methods that can study the action of anycompounds through the GPCRs on cell surface and the consequent events(e.g., trafficking and/or morphological changes) of the effected cellswould be desired. This need and other needs are addressed by the presentinvention.

BRIEF DESCRIPTION OF THE INVENTION

The present invention includes a system and method that uses optical LIDbiosensors to monitor in real time compound-induced mass redistributionincluding agonist-induced GPCR signaling events within living cells.Particularly, the present invention includes a system and method forusing an optical LID biosensor to screen compounds against a target GPCRwithin living cells based on the morphological changes of the celland/or desensitization and/or translocation of the GPCR. In an extendedembodiment, the present invention discloses methods for self-referencingthe optical LID biosensor to eliminate unwanted sensitivity to ambienttemperature, pressure fluctuations, and other environmental changes, andalso methods to provide confirmative information of the compound actionon a particular pre-selected target through comparison of the responsesof two types of cells spatially separated but located on the samesensor. In yet another extended embodiment, the present inventiondiscloses different ways for screening multiple GPCRs in a single typeof cell or multiple GPCRs in multiple types of cells within a singlemedium solution. In still yet another extended embodiment, the presentinvention discloses different ways to confirm the physiological orpharmacological effect of a compound against a specific GPCR withinliving cells.

BRIEF DESCRIPTION OF THE DRAWINGS

A more complete understanding of the present invention may be had byreference to the following detailed description when taken inconjunction with the accompanying drawings wherein:

FIG. 1 is a diagram showing an optical LID system being used to monitora mass redistribution (e.g. GPCR translocation) within a living cell inaccordance with the present invention;

FIG. 2 is a diagram that shows the different states associated with theGPCR translocation within the living cell that can be identified byanalyzing the time dependent optical response output from the opticalLID system shown in FIG. 1 in accordance with the present invention;

FIG. 3 is a flowchart illustrating the basic steps of a method formonitoring in real time an agonist-induced mass redistribution includingGPCR translocation within living cells using an optical LID biosensor inaccordance with the present invention;

FIG. 4 is a flowchart illustrating the basic steps of a method forscreening an agonist against a target GPCR based on mass redistributionwithin living cells using the optical LID biosensor in accordance withthe present invention;

FIG. 5 is a flowchart illustrating the basic steps of a method forscreening an antagonist against a target GPCR based on massredistribution within living cells using the optical LID biosensor inaccordance with the present invention;

FIG. 6 is a flowchart illustrating the basic steps of a method forcreating a self-referencing optical LID biosensor that can be used inany one of the methods shown in FIGS. 3-5 in accordance with the presentinvention;

FIG. 7 is a flowchart illustrating the basic steps of another method forcreating a self-referencing optical LID biosensor that hosts two typesof cells adherent at spatially separated regions within the same sensorand can be used in any one of the methods shown in FIGS. 3-5 inaccordance with the present invention;

FIG. 8 is a flowchart illustrating the basic steps of a method formonitoring an agonist-induced GPCR mass redistribution within multipletypes of living cells using the optical LID biosensor in accordance withthe present invention;

FIG. 9 is a flowchart illustrating the basic steps of a method forscreening agonists against multiple GPCRs within a single type of livingcell based on mass redistribution using the optical LID biosensor inaccordance with the present invention; and

FIGS. 10-17 are various graphs and charts indicating the results ofseveral different experiments that were conducted to show that theoptical LID system can be used to monitor mass redistributions like GPCRtranslocations within living cells that are located on a surface of theoptical LID biosensor in accordance with the present invention. Thisdata was obtained using an optical waveguide grating sensor system andLID microplates (Nb₂O₅ plates), manufactured by Corning Incorporated.

FIG. 10 is a time-dependent LID response of Chinese Hamster Ovary (CHO)cells before and after compound addition.

FIG. 11 is the different kinetics of the mass redistribution due toagonist-induced GPCR activation.

FIG. 12 is the compound-dependant total responses of agonist-inducedmass changes in the Stage 3 as highlighted in FIG. 2.

FIG. 13 is a time-dependent LID response of Chinese Hamster Oyary (CHO)cells before and after compound addition. The compound concentrationused is 10 μM for all compounds.

FIG. 14 a time-dependent LID response of engineered Chinese HamsterOvary (CHO) cells with over-expressed rat muscarnic receptor subtype 1(thus this cell line is termed as M1 CHO) before and after compoundaddition. The compound concentration used is 10 μM for all compounds.

FIG. 15 compares the compound-dependant total responses in the Stage 3as highlighted in FIG. 2 for two distinct cell lines.

FIG. 16 is a time-dependent LID response of two types of cells (CHO andM1 CHO) before and after addition of oxotremorine M (10 μM). Before thecompound addition, the cells are pre-incubated either HBSS buffer(Invitrogen) (referred to “without DIP”) or with dynamin inhibitorypeptide (DIP) at a concentration of 50 μM for 45 minutes.

FIG. 17 is a time-dependent LID response of two types of cells (CHO andM1 CHO) before and after addition of clonidine (10 μM). Before thecompound addition, the cells are pre-incubated either HBSS buffer(Invitrogen) (referred to “without DIP”) or with dynamin inhibitorypeptide (DIP) at a concentration of 50 μM for 45 minutes.

FIG. 18 is a time-dependent LID response of two types of cells (CHO andM1 CHO) before and after addition of NECA (10 μM). Before the compoundaddition, the cells are pre-incubated either HBSS buffer (Invitrogen)(referred to “without DIP”) or with dynamin inhibitory peptide (DIP) ata concentration of 50 μM for 45 minutes.

DETAILED DESCRIPTION OF THE DRAWINGS

Referring to FIG. 1, there is a diagram that shows the basic componentsof an optical LID system 100 which includes an interrogation system 102and an optical LID biosensor 104 that are used to detect and monitor amass redistribution (e.g., the translocation of a GPCR 202, as seen inthe arrow numbered 106) within a living cell 108 (only one shown)located on a surface 110 of the optical LID biosensor 104. In thepreferred embodiment, the interrogation system 102 interrogates theoptical LID biosensor 104 (e.g., SPR sensor 104, waveguide gratingsensor 104) so it can detect and monitor the mass redistribution withinthe living cell 108. This is done by emitting an optical beam 112 whichhas the appropriate spectral or angular content towards the optical LIDbiosensor 104 such that when the optical beam 112 is reflected by thesensing surface 110, the resonant angle or wavelength response whichidentifies the mass redistribution becomes dominant in the reflectedbeam 114. Thus, when there is a detectable mass redistribution withinthe living cell 108, the optical LID biosensor 104 can sense a responsechange which is observed as an angular or wavelength change in thereflected beam 114. The optical response may be recorded as a functionof time. In this way, the kinetics of any event that leads to a massredistribution within the living cell 108 can be analyzed. Prior todiscussing several different types of living cell-based assays that canbe conducted and monitored by the optical LID system 100 (see FIGS.2-18) a detailed discussion is provided about some of the variouscomponents within the living cell 108.

Due to the limited range (˜hundreds nanometers) of the eletromagneticfield propagating in the optical LID sensor 104 that can extend into thesurrounding media (e.g, adherent cell 108) as an evanescenteletromagnetic field (the depth is referred to the penetration depth orsensing volume), only the mass redistribution 106 in the lower portionof the adherent cells that is close to the sensor surface 110 can bedetected. Biological cells 108 are complex structures with componentsranging in size from nanometers to tens of microns. The cell 108 has acytoplasm (10-30 μM) that contains numerous organelles. The largestorganelle is the nucleus, whose size ranges between 3 and 10 μm. Thenucleus is filled with DNA-protein complexes and proteins, the mostimportant one being chromatin. Mitochondria are small organellescomprised of a series of folded membranes with sizes ranging from0.5-1.5 μm. Other cell components include endoplasmic reticulum (ER)(0.2-1 μm), lysomes (0.2-0.5 μm), peroxisomes (0.2-0.5 μm), endosomes(˜100 nm), and gogli. Living cells 108 are highly dynamic and mostorganelles travel extensively within cells. For example, microtubulescan transport organelles over long distances. A stimulus can result inthe submicron movement of densely packed organelles in the veryperiphery of a sensor surface 100 on which the cells 108 are cultured;such movement leads to mass redistribution 106 within the cell 108. Themass redistribution 106 can be detected by an optical biosensor 104; thesignal relating to mass redistribution 106 is referred to as directionalmass redistribution (DMR) signal.

Cellular trafficking could occur if secretory organelles are to occupytheir docking site beneath the plasma membrane, and if endocyticvesicles at the plasma membrane are to reach their processing stationsin the cytosol. In either direction, organelles must penetrate theso-called actin cortex beneath the plasma membrane, a dense meshwork ofactin filaments that is up to a few hundred nanometers thick. To theextent that actin filaments constantly assemble and disassemble, themeshwork is dynamic and permeable to organelles. Control mechanismsregulating the assembly and disassembly would also regulate thepermeability of the actin cortex.

The plasma membrane is a busy place. Exocytic vesicles insert receptorsinto the plasma membrane and release ligands into the extracellularspace. Endocytic vesicles carry receptors with bound ligand to internalprocessing stations. Caveolae are plasma-membrane-associated vesicleswith a presumed role in cell signaling. Lipid rafts are thought topopulate the plasma membrane as small floating islands in which selectmembrane proteins meet in private to exchange signals. Finally, there isthe universe of membrane receptors. Many are probably embedded in largemolecular complexes that continually recruit and release downstreameffector molecules.

Transport of cellular components or extracellular stimuli not onlyoccurs at the plasma membrane, but also occurs at multiple intracellularcompartments. These events include (1) protein target or substraterecruitment to the nucleus, to the membrane, to the cytosol, throughoutrecycling pathways, to or from other organelles, uptake fromextracellular space (ligand binding, gene transfection, infection andprotein delivery); (2) redistribution of newly synthesized intracellularcomponents within various functional compartments at definedmicroenvironments and with mediated release locations. These cellularevents lead to directional mass redistributions at certain times duringsignaling cycles.

From hereinafter, several different types of living cell-based assaysthat can be conducted and monitored by the optical LID system 100 aredescribed in detail below with respect to FIGS. 2-18.

Referring to FIG. 2, there is shown a diagram where the optical LIDsystem 100 is used to monitor an agonist-induced translocation of Gprotein coupled receptors 202 (GPCRs 202) within a living cell 108 (onlyone shown) located on the top surface 110 of the optical LID biosensor104. In particular, the diagram illustrates an agonist induced andtime-dependent optical response 201 that partly is due to thetranslocation of a target GPCR 202 within the living cell 108. The cellis adherent on the top surface 110 of the waveguide-based biosensor 104.For clarity, the interrogation system 102 is not shown in the portionlabeled as “C”.

As can be seen, the GPCR 202 in the resting state resides at the cellsurface 204 (plasma membrane 204), while the GPCR kinase 206 (GRK 206)and arrestin 208 are uniformly distributed inside the living cell 108(see diagram “A”). Upon agonist activation, the GPCR 202 activatesheterotrimeric G proteins composed of α, β, and γ subunits. The Gα andGβγ subunits dissociate which causes the GRK 206 to be recruited to thereceptor 202 at the plasma membrane 204. Then, the GRK 206phosphorylates the carboxy terminus of the GPCR 202. And, β-arrestin208, a relatively abundant intracellular protein, rapidly (withinminutes) translocates within the cytoplasm to the activated GPCR 202 atthe plasma membrane 204, binds the GRK-phosphorylated receptor, anduncouples the receptor from its cognate G protein. The β-arrestin 208then binds to the desensitized GPCR 202 and translocates toclathrin-coated pits at the cell surface 204 where the receptor 202 isinternalized in clathrin-coated vesicles (CCV) (see diagram “B”).Finally, the entire complex 202 and 206 is delivered to the endosome 210(endocytic vesicle 210) (see diagram “C”). This process is known astranslocation. For more information about GPCR translocation, referenceis made to the following three articles:

-   -   Drews, J. “Drug discovery: a historical perspective.” Science        2000, 287, 1960-1963;    -   Ma, P. and Zemmel, R. “Value of novelty”. Nat. Rev. Drug Discov.        2002, 1, 571-572.    -   Pierce, K. L. et al. “Seven-transmembrane receptors.” Nat. Rev.        Mol. Cell Biol. 2002, 3, 639-650.

The contents of these documents are incorporated by reference herein.

It should be appreciated that these translocation events lead todirectional mass distribution (e.g., towards the cell surface or leavingthe cell surface) within the living cells 108 at a certain time,therefore resulting in different optical responses through a prolongperiod of time. Another possible biological event that can lead todirectional mass distribution is the cell morphological changes due tothe GPCR activation. The cell morphological changes involve thecytoskeleton rearrangement as well as cell adhesion changes.Cytoskeleton is a complex network of protein filaments that extendsthroughout the cytoplasm of eucaryotic cells and is involved inexecuting diverse activities in these cells. As well as providingtensile strength for the cells it also enables muscle contraction,carries out cellular movements and is involved in intracellularsignaling and trafficking, cell division and changes in the shape of acell. Activation of G-protein coupled receptors (GPCR) leads to at leasttwo independent events that theoretically could exert an effect on thecytoskeleton rearrangement. The first event is the activation of theintracellular signaling pathway, and the second is a receptor-mediatedendocytosis (i.e., translocation), which occurs after an agonistactivation of the majority of GPCR. Activation of an intracellularsignaling pathway after an agonist/GPCR binding then leads to twofurther sets of connected events. Processes in the first set lead to theactivation of a secondary intracellular signaling pathway (Gprotein→effector→message), while the mechanisms of the second setregulate the degree of signaling within the cell by affecting the eventsin the first set. These mechanisms includephosphorylation/desensitization, internalization and downregulation ofmembrane-bound receptors. It is assumed that both sets of events canlead to the rearrangement of actin filaments within the cell. Forexample, after the activation of GPCR, various forms of G proteins (e.g.G_(α) and G_(βγ)) can bind with F-actin filaments; and those and othersignaling molecules can disassociate from actin filaments. Theinternalization process of membrane-bound receptors that occurs viareceptor-mediated endocytosis could also be responsible for the dynamicsof actin filaments.

Referring again to FIG. 2 and in accordance with the present invention,the different states associated with GPCR translocation within a livingcell 108 can be identified and monitored by analyzing the opticalresponse 201 from the optical LID system 100. In fact, three differentevents can be identified when looking at the optical response 201 shownin FIG. 2. The three major events that can be seen include: (1) a verylarge and sharp decrease in signal 201 upon the addition of agonist, dueto bulk index of refraction changes (i.e., generally the compoundsolution has relatively lower refractive index than the cell medium.Thus compound addition results in a decreased LID signal); (2) atransition stage which has slow changes in the response signal 201 andlasts almost 20 minutes: this stage might be related to thephosphorylation of the activated receptors 202 by GRKs 206, arrestinbinding, desensitization of the receptors 202 to chathrin-coated pits,and/or other cellular responses; and (3) a slow decrease of responsesignal 201 which lasts almost 50 minutes, corresponding to thetranslocation of the GPCR complexes 202 and 208 to the endosome 202. Inother cases, an additional event that immediately followed the initialstep can be evident (e.g., FIG. 11); that is an increase of responsesignal 201, mainly due to diffusion of the compound in the cell mediumand/or recruitment of intracellular components to activated GPCRs atcell surface. Details about how this test can be performed by theoptical LID system 100 are described below with respect to method 300shown in FIG. 3.

Referring to FIG. 3, there is shown a flowchart illustrating the basicsteps of a method 300 for monitoring in real time the massredistribution due to an agonist-induced GPCR activation within livingcells 108 using an optical LID biosensor 104 in accordance with thepresent invention. The method 300 includes the following steps: (a)provide an optical LID biosensor 104 (step 302); (b) place a certainnumber of living cells 108 in a medium which covers the optical LIDbiosensor 104 such that the living cells 108 attach onto the surface 110of the optical LID biosensor 104 (step 304); (c) optionally apply abuffer solution at least once into the cell medium (step 306); (d) applya solution containing a compound (agonist) into the cell medium (step308); and (e) interrogate the optical LID biosensor 104 and monitor thetime dependent optical response 201 of the living cells 108 cultured onthe optical LID biosensor 104 (step 310).

It should be appreciated that if step 306 is performed and a buffersolution (the same buffer solution that is used to formulate thecompound of interest) is applied to the living cells 108 before applyingthe compound, any unwanted effect, due to the living cells 108responding to the environmental changes, can be minimized. This ispossible because living cells 108 that are cultured on the optical LIDbiosensor 104 are alive and dynamic which means that they can sensechanges in the surrounding medium compositions as well as temperatureand can respond to those changes. However, as the living cells 108senses changes like the addition of a buffer then they tend to becomeresistant to those changes in the medium composition assuming noadditional chemical is introduced.

It should also be appreciated that the real time method 300 providesquantifiable information, and equally important, it provides thekinetics of the mass redistribution within cells due to GPCR activation.In contrast to traditional methods of screening GPCRs, this method 300is simpler to perform, more sensitive, label-independent and isapplicable to all GPCRs 202 without requiring prior knowledge of naturalligands or how a given receptor is coupled to downstream signalingpathways.

It should also be appreciated that in the step 304 the number of cellsshould be optimized such that after a certain time cultured underoptimal conditions the cells become adherent and reach high confluency(optionally larger than 75%) on the surface 110 of optical LID sensor104 in order to achieve high sensitivity.

Referring to FIG. 4, there is shown a flowchart illustrating the basicsteps of a method 400 for screening an agonist against a target GPCR 202based on mass redistribution within living cells 108 using the opticalLID biosensor 104 in accordance with the present invention. The method400 includes the following steps:

(a) provide the optical LID biosensor 104 (step 402); (b) place acertain number of living cells 108 in a medium which covers the opticalLID biosensor 104 such that the living cells 108 attach onto the surface110 of the biosensor 104 (step 404); (c) apply a solution containing anantagonist with a known affinity at a certain concentration into thecell medium for a certain time until the optical LID biosensor 104becomes stabilized (step 406); (d) apply a solution containing acompound (agonist) into the cell medium (step 408) where theconcentration of the compound is sufficiently high to compete off thereceptor-bound antagonist; and (e) interrogate the optical LID biosensor104 and monitor the time dependent optical response 201 of the livingcells 108 cultured on the optical LID biosensor 104 (step 410).

It should be appreciated that in this method 400 by pre-applying theantagonist to one receptor in the living cells 108, effectively enablesone to screen the compounds for their agonism against this particularreceptor. Moreover, it should be appreciated that this method 400 issimilar to the previous method 300 except for one difference in thatmethod 400 requires pre-knowledge about the functionality of thecompound for its cognate receptor in the living cells 108. For instance,one needs to know whether the antagonist inhibits the activation of GPCR202, or whether the antagonist activates the GPCR 200 which leads totranslocation.

Referring to FIG. 5, there is shown a flowchart illustrating the basicsteps of a method 500 for screening an antagonist against a target GPCR202 based on mass redistribution within living cells 108 using theoptical LID biosensor 104 in accordance with the present invention. Themethod 500 includes the following steps:

(a) provide an optical LID biosensor 104 (step 502); (b) place a certainnumber of living cells 108 in a medium which covers the optical LIDbiosensor 104 such that the living cells 108 attach onto the surface 110of the biosensor 104 (step 504); (c) apply a solution containing anagonist which has a known affinity at a certain concentration into thecell medium for a short time such that the translocation does not happen(step 506); (d) after this short time, apply a solution containing acompound having a certain concentration into the cell medium (step 508);and (e) interrogate the optical LID biosensor 104 and monitor the timedependent optical response 201 of the living cells 108 cultured on theoptical LID biosensor 104. It should be appreciated that like method400, this method 500 requires pre-knowledge about the target GPCR 202 inthe living cells 108 and also requires the pre-selection of anantagonist or angonist for pre-treating the living cell 108 against thisparticular GPCR 202.

It should be appreciated that the step 506 and the step 508 can becombined into one step; that is, the agonist known to the target GPCR inthe cell can be added into together with a compound. It also should beappreciated that similar to the method 300, the compound to be testedcan be introduced first, followed by the addition of the known ofagonist.

Each of the methods 300, 400 and 500 can be further enhanced by using aself-referencing optical LID biosensor 104. It is well known that theperformance of the optical LID biosensor 104 is generally affected bythe designs and characteristics of the sensor, the optics, and by theenvironmental fluctuations including ambient temperature and pressure. Amain advantage of using the self-referencing optical LID biosensor 104is that the top surface 110 has both a reference region and a sampleregion which enables one to use the sample region to detect the massredistribution in the living cells 108 and at the same time use thereference region which does not have living cells 108 attached theretoto reference out spurious changes that can adversely affect thedetection of the mass redistribution within the living cells 108.

In one embodiment, the self-referencing optical LID biosensor 104 can bemade in accordance with method 600 shown in FIG. 6. Thisself-referencing optical LID biosensor 104 can be created by using thefollowing steps: (a) provide the optical LID biosensor 104 (step 602);(b) physically block one region (reference region) of the surface 110 ofthe optical LID biosensor 104 by using a soft stamp (e.g., rubber stamp)(step 604); (c) place a certain number of living cells in a growthmedium which covers an unblocked region (sample region) of the opticalLID biosensor 104 (step 606); and (d) remove the soft stamp after theliving cells 108 have attached to the unblocked region on the opticalLID biosensor 104 (step 608). At this point, the living cell-based assaycan be performed as described in methods 300, 400 and 500. It should beappreciated that different methods can also be applied to create theself-referencing LID sensors for cell studies. For example, a physicalbarrier can be used to divide the sensor into two portions, and cells ina medium are only applied to cover one portion. After cell adhesion, thephysical barrier can be removed.

Referring now to another feature of the present invention, it is wellknown that multiplexed cell assays have become increasingly important,not only for increasing throughput, but also for the rich andconfirmative information available from a single assay. As such, it isdesirable if the present invention could be further enhanced to performmultiple living cell-based assays at the same time.

In one embodiment, the present invention can be enhanced to performmultiple living cell-based assays at the same time by using the method700 shown in FIG. 7. In accordance with method 700 one can monitor massredistribution due to agonist-induced GPCR activation within multipletypes of the living cells 108 by: (a) providing an optical LID biosensor104 (step 702); (b) blocking a portion of the top surface 110 of theoptical LID biosensor 104 by using a stamp that prevents the attachmentof the living cells 108 to that portion of the optical LID biosensor 104(step 704); (c) placing a first type of living cells 108 in a cellmedium which covers the unblocked portion of the surface 110 of theoptical LID biosensor 104 so the living cells 108 are able to attach tothe unblock portion of the surface 110 of the optical LID biosensor 104(step 706); (d) removing the stamp from the top surface 110 of theoptical LID biosensor 104 (step 708); (e) placing a second type ofliving cells 108 in a cell medium which covers the optical LID biosensor104 so the second type of living cells 108 are able to attach to therecently uncovered top surface 110 of the optical LID biosensor 104(step 710); (f) applying a solution containing a compound into the cellmedium located on the top surface 110 of the optical LID biosensor 104(step 712); and (g) interrogating the optical LID biosensor 104 tomonitor time dependent optical responses 201 which indicate massredistributions within the two types of living cells 108 on the opticalLID biosensors 104 (step 714).

It should be appreciated that the two types of cells can be related;e.g., Chinese Hamster Ovary (CHO) cells versus engineered CHO cellscontaining an overexpressed target receptor. This approach not onlyenables multiplexed cell assays, but also provide confirmative resultsregarding to the compound effect on the target receptor by comparison ofthe optical responses of the same compound acting on two differentcells, since two cells are identical except for the target receptorexpression level.

In another embodiment, the present invention can be enhanced to performmultiple living cell-based assays at the same time using the method 800shown in FIG. 8. In accordance with method 800 one can monitor the massredistribution due to agonist-induced GPCR activation in multiple typesof living cells 108 by: (a) providing a chamber (microplate) containingan array of the optical LID biosensors 104 (step 802); (b) placing afirst type of living cells 108 in a cell medium which covers one or moreof the optical LID biosensors 104 so the first type of living cells 108are able to attach to the surfaces 110 of the one or more optical LIDbiosensors 104 (step 804); (c) placing a second type of living cells 108in a cell medium which covers one or more of the remaining uncoveredoptical LID biosensors so the second type of living cells 108 are ableto attach to the surfaces 110 of the one or more remaining uncoveredoptical LID biosensors 104 (step 806); (d) applying a solutioncontaining a compound into the cell mediums located on the top surfaces110 of covered optical LID biosensors 104 (step 810); and (e)interrogating the covered optical LID biosensors 110 to monitor the timedependent optical responses 201 which indicate mass redistributionswithin the living cells 108 on each of the covered optical LIDbiosensors 104 (step 812).

It should be appreciated that arrays of different DNA vectors containingdistinct target receptor genes in combination with transfection reagentscan be deposited onto a LID sensor; a single type of cells is placed andoverlaid with such array and uptakes the genes. Thus only cells overlaidon each spot area become transfected and therefore forming a transfectedcell cluster array (U.S. Pat. No. 6,544,790 B1 “Reverse transfectionmethod”). Similarly, array of functional receptor proteins in complexedwith protein delivery reagents can be used to similar transfected cellcluster array (US2004/0023391A1 “Methods and devices for proteindelivery”). Both types of transfected cell arrays can be used forcompound screening using the current technology.

In yet another embodiment, the present invention can be further enhancedto perform multiple target screens in a single type of cellsat the sametime by using method 900 shown in FIG. 9. In accordance with method 900one can screen agonists against multiple GPCRs 202 within a single typeof living cells 108 by performing the following steps: (a) providing aoptical LID biosensor 104 (step 902); (b) placing the living cells 108in a cell medium which covers the optical LID biosensor 104 so theliving cells 108 are able to attach to the surface 110 of the opticalLID biosensor 104 (step 904); (c) applying a solution containing acocktail solution of antagonists (step 906); (d) applying a solutioncontaining a compound into the cell medium located on the top surface110 of the optical LID biosensor 104 (step 908); and (e) interrogatingthe optical LID biosensor 104 to monitor a time dependent opticalresponse 201 which indicates mass redistributions within the livingcells 108 (step 910).

It should be appreciated that similar method can be used to screenantagonist against multiple receptors in the same cell line by modifyingthe method 900. Instead of a cocktail solution of antagonists in thestep 906, one can use a solution of compounds of interest; at the sametime, a cocktail solution of agonists is used to replace the compoundsolution in the step 908.

Following is a discussion about the results of several differentexperiments that were conducted to show that an optical LID system 100can be used to monitor a mass redistribution within living cells 108that are located on the surface 110 of the optical LID biosensor 104(see FIG. 2).

FIG. 10 is a graph that shows several agonist-induced responses withinchinese hamster ovary cells 108 (CHO 108) that were monitored by theoptical LID system 100. It is known that CHO cells 108 endogenouslyexpress beta adrenergic receptors, alpha2-adrenergic receptors, P2Yreceptors, as well as beta-arrestin and GRKs. It is also known thatmuscarinic receptors are endogenously expressed at very low level in theCHO cells 108. In this experiment, approximately ˜5×10⁴ CHO cells 108were placed within each well of a microplate that contained an array ofoptical LID biosensors 104. The CHO cells 108 were then cultured in 150μl serum medium for 24 hours to ensure that the CHO cells 108 becameadherent to the substrate surface 110.

The graph shows the optical responses of the CHO cells 108 to fourdifferent compounds which were examined: (1) ATP (100 μM), agonist forP2Y receptors; (2) clonidine (10 μM), agonist for alpha2-adrenergicreceptors; (3) epinephrine (100 μM), agonist for beta adrenergicreceptors; and (4) oxotremorine M (10 μM), agonist for muscarinicreceptors. Since muscarinic receptors are endogenously expressed at verylow level in CHO cells 108, oxotremorine M, agonist for muscarinicreceptors, served as a control. Each of these agonists was directlyapplied to a different one of the wells which contained the serummedium. The optical responses were then collected by the optical LIDsystem 100.

The results showed that these adherent CHO cells 108 gave rise tosimilar kinetics and transitions as shown by the optical responses afterthe introduction of the three agonists: ATP, clonidine, and epinephrine.Oxotremorine M caused almost no cell response. In FIG. 11, a kineticsanalysis of the later stage of the process revealed that all threeagonists (ATP, epinephrine, clonidine) resulted in a similar slowprocess. The changes for the Stage 3 as shown in FIG. 2, caused by thoseagonists, are shown in the graph in FIG. 12. The similar changes mightreflect the fact that beta-arrestin, a critical component for GPCRtranslocation, could be the limiting factor in the CHO cells 108, giventhat the size of clathrin-coated pits and beta-arrestin are similar.

FIG. 13 is a graph that shows the results from an experiment whichindicates the ligand- and time-dependent response of a monolayer ofliving CHO cells 108 on wave-guide biosensors 104. The agonists whichwere used included: (1) clonidine; (2) oxotremorine M; (3) NECA; and antelenzepine, an antagonist for M1 receptor, is also used.

FIG. 14 is a graph that shows the results from an experiment whichindicates the ligand- and time-dependent response of monolayer of livingCHO cells 108 with stably overexpressed rat muscarinic receptor subtype1 (M1) on wave-guide biosensors 104. The agonists which were usedincluded: (1) clonidine; (2) oxotremorine M; and (3) NECA; and antelenzepine, an antagonist for M1 receptor, is also used.

FIG. 15 is a graph that shows the results from an experiment whichindicates the ligand-induced total change in response of monolayer ofliving CHO cells 108 without (CHO) and with stably overexpressed ratmuscarinic receptor subtype 1(M1CHO) on wave-guide biosensors 104.Results shown in FIGS. 11 and 13-15 indicated that (1) there are alpha2adrenergic receptors expressed in both CHO and M1-CHO cells; and theiragonist (clonidine) induced mass redistribution signals; (2) there isrelatively low or almost no M1 receptor expressed in CHO cells, but highin M1-CHO cells since its agonist (oxotremorine M) but not itsantagonist (telenzepine) results in significantly larger responses inM1-CHO cells.

FIG. 16 is a graph that shows the results from an experiment whichindicates the effect of pre-incubation of a dynamin phosphorylationinhibitor (dynamin inhibitory peptide, DIP) on oxotremorine M-inducedtime-dependent response of a monolayer of living CHO cells 108 withoutand with stably overexpressed rat muscarinic receptor subtype 1 (M1CHO)on wave-guide biosensors 104. Results show that the pre-incubation ofcells with DIP almost totally eliminates the oxotremorine M-induced massdistribution responses in both cell lines, suggesting that oxotremorineM-induced mass distribution is dynamin-dependent. The dynamin-dependencyis common for most of agonist-induced GPCR translocation.

FIG. 17 is a graph that shows the results from an experiment whichindicates the effect of pre-incubation of a dynamin phosphorylationinhibitor (dynamin inhibitory peptide, DIP) on clonidine-inducedtime-dependent response of a monolayer of living Chinese Hamster Ovary(CHO) cells without and with stably overexpressed rat muscarinicreceptor subtype 1 (M1CHO) on wave-guide biosensors 104. Results showthat the pre-incubation of both cell lines with DIP almost totallyeliminates the clonidine-induced mass distribution response, suggestingthat clonidine-induced mass distribution is also dynamin-dependent.

FIG. 18 is a graph that shows the results from an experiment whichindicates the effect of pre-incubation of a dynamin phosphorylationinhibitor (dynamin inhibitory peptide, DIP) on NECA-inducedtime-dependent response of a monolayer of living CHO cells 108 withoutand with stably overexpressed rat muscarinic receptor subtype 1 (M1CHO)on wave-guide biosensors 104. Results showed that the pre-incubation ofboth cells with DIP has little effect on NECA-induced response,suggesting that NECA results in mass redistribution signals in both celllines through a dynamin-independent pathway.

Some additional features and advantages of using the optical LID system100 of the present invention are as follows:

(1) The present invention discloses a real time method that can be usedto perform a label free functional GPCR cell-based assay which enablescompound screening and profiling. This method allows one to study anendogenous but relatively highly expressed GPCR in living cells withoutneeding to genetically engineer the cell to over-express a receptor ofinterest.

(2) The present invention discloses methods to perform multiplexedcell-based assays using a single sensor which offers an advantage ofincreased throughput.

(3) The preferred optical LID biosensor 104 is a SPR sensor 104 or awaveguide grating based sensor 104. Other optical-based biosensors canalso be used such as ellipsometry devices, evanescent wave devices, andreflectometry devices. For a more detailed discussion about thestructure and operation of these two types of optical LID biosensors 104reference is made to the following documents:

-   -   U.S. Pat. No. 4,815,843 entitled “Optical Sensor for Selective        Detection of Substances and/or for the Detection of Refractive        Index Changes in Gaseous, Liquid, Solid and Porous Samples”.    -   K. Tiefenthaler et al. “Integrated Optical Switches and Gas        Sensors” Opt. Lett. 10, No. 4, April 1984, pp. 137-139.        The contents of these documents are incorporated by reference        herein.

Although several embodiments of the present invention have beenillustrated in the accompanying Drawings and described in the foregoingDetailed Description, it should be understood that the invention is notlimited to the embodiments disclosed, but is capable of numerousrearrangements, modifications and substitutions without departing fromthe spirit of the invention as set forth and defined by the followingclaims.

1. A method for performing a living cell-based assay, said methodcomprising the step of: using an optical label independent detection(LID) biosensor to monitor mass redistribution within living cellsadherent on a surface of the optical LID biosensor.
 2. The method ofclaim 1, wherein said step of using enables one to monitor massredistribution due to an agonist-induced G-protein coupled receptor(GPCR) activation within living cells by performing the following steps:providing the optical LID biosensor; placing the living cells in a cellmedium to cover the optical LID biosensor so the living cells are ableto attach to the surface of the optical LID biosensor; applying asolution containing a compound into the cell medium located on thesurface of the optical LID biosensor; and interrogating the optical LIDbiosensor to obtain a time dependent optical response which indicatesthe mass redistribution within the living cells that enables one tomonitor an agonist-induced GPCR translocation within the living cells.3. The method of claim 2, wherein prior to the step of applying thesolution containing the compound into the cell medium located on thesurface of the optical LID biosensor the following step is performed:applying a buffer solution at least once into the cell medium located onthe surface of the optical LID biosensor solution.
 4. The method ofclaim 1, wherein said step of using enables one to screen an agonistagainst a target G-protein coupled receptor (GPCR) based on massredistribution within living cells by performing the following steps:providing the optical LID biosensor; placing the living cells in a cellmedium to cover the optical LID biosensor so the living cells are ableto attach to the surface of the optical LID biosensor; applying asolution containing an antagonist with a known affinity at a certainconcentration into the cell medium to stabilize the optical LIDbiosensor; applying a solution containing a compound into the cellmedium located on the surface of the optical LID biosensor, wherein aconcentration of the compound is sufficiently high to compete off theantagonist; and interrogating the optical LID biosensor to obtain a timedependent optical response which indicates the mass redistributionwithin the living cells that enables one to screen an agonist against atarget GPCR based on mass redistribution within the living cells.
 5. Themethod of claim 1, wherein said step of using enables one to screen anantagonist against a target G-protein coupled receptor (GPCR) based onmass redistribution within living cells by performing the followingsteps: providing the optical LID biosensor; placing the living cells ina cell medium to cover the optical LID biosensor so the living cells areable to attach to the surface of the optical LID biosensor; applying asolution containing an agonist with a known affinity at a certainconcentration into the cell medium located on the surface of the opticalLID biosensor; applying, before translocation happens due to thepresence of the agonist, a solution containing a compound into the cellmedium located on the surface of the optical LID biosensor; andinterrogating the optical LID biosensor to obtain a time dependentoptical response which indicates the mass redistribution within theliving cells that enables one to screen an antagonist against a targetGPCR based on mass redistribution within living cells.
 6. The method ofclaim 1, wherein said optical LID biosensor is formed into aself-referencing optical LID biosensor prior to performing the step ofusing by: blocking a portion of the surface of the optical LID biosensorusing a stamp that prevents attachment of the living cells; placing theliving cells in a cell medium to cover an unblocked portion of thesurface of the optical LID biosensor so the living cells are able toattach to the unblocked portion of the surface of the optical LIDbiosensor; and removing the stamp from the surface of the optical LIDbiosensor.
 7. The method of claim 1, wherein said optical LID biosensoris formed into a self-referencing optical LID biosensor prior toperforming the step of using by: blocking a portion of the surface ofthe optical LID biosensor using a stamp that prevents attachment of theliving cells; placing the living cells in a cell medium to cover anunblocked portion of the surface of the optical LID biosensor so theliving cells are able to attach to the unblocked portion of the surfaceof the optical LID biosensor; removing the stamp from the surface of theoptical LID biosensor, and and placing a second type of cells in thesame cell medium to cover the blocked portion of the surface of theoptical LID biosensor so the second type of cells are able to attach tothe blocked portion of the surface of the optical LID biosensor.
 8. Themethod of claim 1, wherein said step of using optical LID sensor enablesone to monitor the mass redistribution due to an agonist-inducedG-protein coupled receptor (GPCR) activation within multiple types ofthe living cells by performing the following steps: providing a chambercontaining an array of the optical LID biosensors; placing a first typeof the living cells in a cell medium to cover at least one of theoptical LID biosensors so the first type of the living cells is able toattach to the surface of the at least one optical LID biosensor; placinga second type of the living cells in a cell medium to cover at least oneof the remaining uncovered optical LID biosensors so the second type ofthe living cells is able to attach to the surface of the at least oneremaining uncovered optical LID biosensor; applying a solutioncontaining a compound into the cell mediums located on the surfaces ofthe optical LID biosensors; and interrogating the optical LID biosensorsto obtain time dependent optical responses which indicates the massredistributions within the living cells on each of the optical LIDbiosensors that enables one to monitor the agonist-induced GPCRactivation within multiple types of the living cells.
 9. The method ofclaim 1, wherein said step of using optical LID sensor enables one tomonitor mass redistribution due to an agonist-induced G-protein coupledreceptor (GPCR) activation within multiple types of the living cells byperforming the following steps: providing the optical LID biosensor;blocking a portion of the surface of the optical LID biosensor using astamp that prevents attachment of the living cells to that portion ofthe optical LID biosensor; placing a first type of the living cells in acell medium to cover an unblocked portion of the surface of the opticalLID biosensor so the first type of the living cells are able to attachto the unblocked portion of the surface of the optical LID biosensor;removing the stamp from the surface of the optical LID biosensor;placing a second type of the living cells in a cell medium to cover theoptical LID biosensor so the second type of the living cells are able toattach to the uncovered portion of the surface on the optical LIDbiosensor; applying a solution containing a compound into the cellmedium located on the surface of the optical LID biosensor; andinterrogating the optical LID biosensors to obtain time dependentoptical responses which indicates the mass redistributions within thetwo types of living cells on the optical LID biosensor that enables oneto monitor the agonist-induced GPCR activation within multiple types ofthe living cells.
 10. The method of claim 1, wherein said step of usingoptical LID sensor enables one to screen agonists against multipleG-protein coupled receptors (GPCRs) within a single type of living cellsby performing the following steps: providing the optical LID biosensor;placing the living cells in a cell medium to cover the optical LIDbiosensor so the living cells are able to attach to the surface of theoptical LID biosensor; applying a solution containing a cocktailsolution of antagonists; applying a solution containing a compound intothe cell medium located on the surface of the optical LID biosensor; andinterrogating the optical LID biosensor to obtain a time dependentoptical response which indicates the mass redistribution within theliving cells that enables one to screen agonists against multiple GPCRswithin the living cells.
 11. A system, comprising: an interrogationsystem; and an optical label independent detection (LID) biosensor,wherein said interrogation system emits an optical beam to said opticalLID biosensor and receives an optical beam from said optical LIDbiosensor which enables said interrogation system to monitor massredistribution within living cells located on a surface of the opticalLID biosensor.
 12. The system of claim 11, wherein said interrogationsystem is further capable of monitoring an agonist-induced G proteincoupled receptor (GPCR) activation within the living cells after thefollowing steps are performed: providing the optical LID biosensor;placing the living cells in a cell medium to cover the optical LIDbiosensor so the living cells are able to attach to the surface of theoptical LID biosensor; applying a solution containing a compound intothe cell medium located on the surface of the optical LID biosensor; andinterrogating the optical LID biosensor to obtain a time dependentoptical response which indicates the mass redistribution within theliving cells that enables one to monitor the mass redistribution due toan agonist-induced GPCR activation within the living cells.
 13. Thesystem of claim 12, wherein prior to the step of applying the solutioncontaining the compound into the cell medium located on the surface ofthe optical LID biosensor the following step is performed: applying abuffer solution at least once into the cell medium located on thesurface of the optical LID biosensor solution.
 14. The system of claim11, wherein said interrogation system is further capable of screening anagonist against a target G-protein coupled receptor (GPCR) based on massredistribution after the following steps are performed: providing theoptical LID biosensor; placing the living cells in a cell medium tocover the optical LID biosensor so the living cells are able to attachto the surface of the optical LID biosensor; applying a solutioncontaining an antagonist with a known affinity at a certainconcentration into the cell medium to stabilize the optical LIDbiosensor; applying a solution containing a compound into the cellmedium located on the surface of the optical LID biosensor, wherein aconcentration of the compound is sufficiently high to compete off theantagonist; and interrogating the optical LID biosensor to obtain a timedependent optical response which indicates the mass redistributionwithin the living cells that enables one to screen an agonist against atarget GPCR based on mass redistribution within the living cells. 15.The system of claim 11, wherein said interrogation system is furthercapable of screening an antagonist against a target G-protein coupledreceptor (GPCR) based on mass redistribution after the following stepsare performed: providing the optical LID biosensor; placing the livingcells in a cell medium to cover the optical LID biosensor so the livingcells are able to attach to the surface of the optical LID biosensor;applying a solution containing an agonist with a known affinity at acertain concentration into the cell medium located on the surface of theoptical LID biosensor; applying, before translocation happens due to thepresence of the agonist, a solution containing a compound into the cellmedium located on the surface of the optical LID biosensor; andinterrogating the optical LID biosensor to obtain a time dependentoptical response which indicates the mass redistribution within theliving cells that enables one to screen an antagonist against a targetGPCR based on mass redistribution within living cells.
 16. The system ofclaim 11, wherein said optical LID biosensor is formed into aself-referencing optical LID biosensor by: blocking a portion of thesurface of the optical LID biosensor using a stamp that preventsattachment of the living cells; placing the living cells in a cellmedium to cover an unblocked portion of the surface of the optical LIDbiosensor so the living cells are able to attach to the unblockedportion of the surface of the optical LID biosensor; and removing thestamp from the surface of the optical LID biosensor.
 17. The system ofclaim 11, wherein said optical LID biosensor is formed into aself-referencing optical LID biosensor prior to performing the step ofusing by: blocking a portion of the surface of the optical LID biosensorusing a stamp that prevents attachment of the living cells; placing theliving cells in a cell medium to cover an unblocked portion of thesurface of the optical LID biosensor so the living cells are able toattach to the unblock portion of the surface of the optical LIDbiosensor; removing the stamp from the surface of the optical LIDbiosensor, and and placing a second type of cells in the same cellmedium to cover the blocked portion of the surface of the optical LIDbiosensor so the second type of cells are able to attach to the blockedportion of the surface of the optical LID biosensor.
 18. The system ofclaim 11, wherein said interrogation system is further capable ofmonitoring the mass redistribution due to an agonist-induced G-proteincoupled receptor (GPCR) activation within multiple types of the livingcells after the following steps are performed: providing a chambercontaining an array of the optical LID biosensors; placing a first typeof the living cells in a cell medium to cover at least one of theoptical LID biosensors so the first type of the living cells is able toattach to the surface of the at least one optical LID biosensor; placinga second type of the living cells in a cell medium to cover at least oneof the remaining uncovered optical LID biosensors so the second type ofthe living cells is able to attach to the surface of the at least oneremaining uncovered optical LID biosensor; applying a solutioncontaining a compound into the cell mediums located on the surfaces ofthe optical LID biosensors; and interrogating the optical LID biosensorsto obtain time dependent optical responses which indicates the massredistributions within the living cells on each of the optical LIDbiosensors that enables one to monitor the agonist-induced GPCRtranslocation within multiple types of the living cells.
 19. The systemof claim 11, wherein said interrogation system is further capable ofmonitoring the mass redistribution due to an agonist-induced G-proteincoupled receptor (GPCR) activation within multiple types of the livingcells after the following steps are performed: providing the optical LIDbiosensor; blocking a portion of the surface of the optical LIDbiosensor using a stamp that prevents attachment of the living cells tothat portion of the optical LID biosensor; placing a first type of theliving cells in a cell medium to cover an unblocked portion of thesurface of the optical LID biosensor so the first type of the livingcells are able to attach to the unblock portion of the surface of theoptical LID biosensor; removing the stamp from the surface of theoptical LID biosensor; placing a second type of the living cells in acell medium to cover the optical LID biosensor so the second type of theliving cells are able to attach to the uncovered portion of the surfaceon the optical LID biosensor; applying a solution containing a compoundinto the cell medium located on the surface of the optical LIDbiosensor; and interrogating the optical LID biosensors to obtain timedependent optical responses which indicates the mass redistributionswithin the two types of living cells on the optical LID biosensor thatenables one to monitor the agonist-induced GPCR translocations withinmultiple types of the living cells.
 20. The system of claim 11, whereinsaid interrogation system is further capable of screening agonistsagainst multiple G-protein coupled receptors (GPCRs) within a singletype of living cells after the following steps are performed: providingthe optical LID biosensor; placing the living cells in a cell medium tocover the optical LID biosensor so the living cells are able to attachto the surface of the optical LID biosensor; applying a solutioncontaining a cocktail solution of antagonists; applying a solutioncontaining a compound into the cell medium located on the surface of theoptical LID biosensor; and interrogating the optical LID biosensor toobtain a time dependent optical response which indicates the massredistribution within the living cells that enables one to screenagonists against multiple GPCRs within the living cells.